Our Freeze-Substitution Method for Histochemical Studies
نویسندگان
چکیده
منابع مشابه
Epoxy resin as fixative during freeze-substitution.
An alternative protocol for freeze-substitution is described. Araldite/Epon embedding medium (20% in acetone) is first used as a stabilizer (as e.g., OsO(4)) and then as embedding medium. The major components of the Araldite/Epon resin formulation react with proteins and lipids and provide for an excellent preservation and reasonable visualisation of the ultrastructure. The ultrastructural appe...
متن کاملApplications of Freeze-substitution to Electron Microscope Studies of Invertebrate Oocytes
A modified freeze-substitution process is described which gives a low percentage (less than 5 per cent) of preparations of invertebrate eggs which appear to be ice crystal-free at the resolution of the electron microscope. The mitochondria show no membranes in these preparations but can be recognized by internal spaces with the size and the distribution of the cristae. The Golgi bodies resemble...
متن کاملStructure of dictyosomes in Scenedesmus acuminatus observed by the freeze-substitution method.
The ultrastructure of dictyosomes in Scenedesmus acuminatus was studied by the freeze-substitution method. The cisternal membranes of dictyosomes clearly exhibited three-layered structures. Electron-dense particles were visible on or in the proximal cisternal membranes. The upper and lower membranes of the distal cisternae were in close contact with each other at the cisternal centre. The elect...
متن کاملMethods and Principles of Fixation by Freeze-Substitution
Freeze-substitution is based on rapid freezing of tissues followed by solution ("substitution") of ice at temperatures well below O degrees C. A 1 to 3 mm. specimen was thrown into 3:1 propane-isopentane cooled by liquid nitrogen to -175 degrees C. (with precautions). The frozen tissue was placed in substituting fluid at -70 degrees C. for 1 week to dissolve ice slowly without distorting tissue...
متن کاملHistochemical studies in atherogenesis. Human cerebral arteries.
THE HISTOLOGY and histochemistry of cerebral arteries have been subject to only a few investigations. Duff, Forbus, Glynn, and Carmichaelhave stressed the importance of the integrity of the internal elastic membrane. Wolkoff studied the relationship between the elastic elements and ground substance components in cerebral arteries. He noted that lipid was occasionally seen between the reduplicat...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the Japanese Histochemical Association
سال: 1963
ISSN: 1883-9444
DOI: 10.1267/ahc1960.1963.37